LSM for unequal sequence 2x2

[scarvajal]

Hi all, for 2x2 study, someone know if WinNonlin calculate Least Square Means(LSM) assuming equal sequence 2x2 (n1= n2) or distingue when exist inequal sequence (n1=10, n2=18)?

Thanks,
SC

[Helmut_Schuetz]

Hi Susana,

the latter. No problems with imbalanced sequences in all versions of WinNonlin (classic) and Phoenix/WinNonlin. Also no problem with unequal group sizes in parallel designs:

• Schütz H, Labes D, and A Fuglsang
  Reference Datasets for 2-Treatment, 2-Sequence, 2-Period Bioequivalence Studies
  AAPS J 16(6), 1292–7 (2014)
  doi 10.1208/s12248-014-9661-0 PMID: 25212768
• Fuglsang A, Schütz H, and D Labes
  Reference Datasets for Bioequivalence Trials in a Two-Group Parallel Design
  AAPS J (Epub 9 December 2014)
  doi 10.1208/s12248-014-9704-6 PMID: 25488055

[scarvajal]

Thank you very much for your answer,

SC